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Independent Recommendation Letter Sample #3 (PDF), EB-2 NIW

Updated: Author: Alina Kanametova

Template Templates 3 pages Anonymized Access on request

Independent recommendation letter template #3 for I-140 EB-2 NIW: a third 3-page variant of the outside-expert letter structure.

O-1 EB-1A EB-2 NIW Recommendation letters Petition

About this document

An independent recommendation letter for an EB-2 NIW petition that supports the applicant’s research profile in bioprocessing and enzyme production. It highlights three published studies on Aspergillus nidulans, growth control, melanin reduction, and trickle bed reactor experiments, and notes more than seventy citations worldwide. The letter also says the applicant has four additional peer-reviewed papers on butanol and ethanol fermentation.

Key points

  • Recommender says he knows the applicant only through published work and has not met or worked with him.
  • The letter focuses on published research in bioprocessing, especially enzyme production with Aspergillus nidulans.
  • One paper used pyridoxine limitation to control fungal growth in a novel bioreactor system and avoid clogging.
  • The applicant reduced growth by 95% while maintaining enzyme production in scale-down experiments.
  • A second study cut melanization by adjusting zinc and copper and adding ascorbic acid, doubling enzyme production.
  • A third study used a 1.1L trickle bed reactor, different packing materials, and tested dilution and air flow effects.
  • The letter says the research papers have been cited more than seventy times by researchers in multiple countries.
  • It also mentions four additional peer-reviewed papers on butanol and ethanol fermentation.

What is inside

  1. Opening support statement
  2. Recommender expertise and independence
  3. Article 1 on growth-limited enzyme production
  4. Article 2 on melanin control in TBR
  5. Article 3 on continuous production in 1.1L TBR
  6. Additional publications and citation count
  7. Closing endorsement

When it helps

Supports the evidence section of an EB-2 NIW file by showing an independent recommender describing the applicant's published research, technical originality, and citation impact. It also serves as an outside expert opinion on the significance of the applicant's bioprocessing work.

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